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In vitro selection has been an essential tool in the development of recombinant antibodies against various antigen targets. Deep sequencing has recently been gaining ground as an alternative and valuable method to analyze such antibody selections. The analysis provides a novel and extremely detailed view of selected antibody populations, and allows the identification of specific antibodies using only sequencing data, potentially eliminating the need for expensive and laborious low-throughput screening methods such as enzyme-linked immunosorbant assay.
The high cost and the need for bioinformatics experts and powerful computer clusters, however, have limited the general use of deep sequencing in antibody selections. Here, we describe the AbMining ToolBox, an open source software package for the straightforward analysis of antibody libraries sequenced by the three main next generation sequencing platforms , Ion Torrent, MiSeq.
The ToolBox is able to identify heavy chain CDR3s as effectively as more computationally intense software, and can be easily adapted to analyze other portions of antibody variable genes, as well as the selection outputs of libraries based on different scaffolds. The software runs on all common operating systems Microsoft Windows, Mac OS X, Linux , on standard personal computers, and sequence analysis of 1—2 million reads can be accomplished in 10—15 min, a fraction of the time of competing software.
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The region was created by the territorial reform of French Regions in through the merger of three regions: Aquitaine , Limousin and Poitou-Charentes. The new region was established on 1 January , following the regional elections in December It is the largest region in France by area, with a territory slightly larger than that of Austria ; even French Guiana is smaller. Its largest city, Bordeaux , together with its suburbs and satellite cities, forms the 7th-largest metropolitan area of France, with , inhabitants.
The region has 25 major urban areas, among which the most important after Bordeaux are Bayonne , inhabitants , Limoges , , Poitiers , , Pau , , and La Rochelle , , as well as 11 major clusters. The agricultural region of Europe with the greatest turnover, it is the French region with the most tourism jobs, as it has three of the four historic resorts on the French Atlantic coast: Arcachon , Biarritz and Royan , as well as several ski resorts e.
Gourette , and is the fifth French region for business creation all sectors. Its economy is based on agriculture and viticulture vineyards of Bordeaux and Cognac , tourism , a powerful aerospace industry , digital economy and design, parachemical and pharmaceutical industries, financial sector Niort is the fourth-largest financial center in the nation, specialising in mutual insurance companies , and industrial ceramics Limoges.
Historically, it is the “indirect successor” to medieval Aquitaine , and extends over a large part of the former Duchy of Eleanor of Aquitaine. The region’s interim name Aquitaine-Limousin-Poitou-Charentes was a hyphenated placename , known as ALPC, created by hyphenating the merged regions’ names — Aquitaine , Limousin and Poitou-Charentes — in alphabetical order. The other popular favourite, “Grande Aquitaine,” was rejected for its connotation with a feeling of superiority.
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